Table 2.

Oligonucleotide sequences for IL-8 HP with different stem-loop structures

Designation	Sequence (5′ to 3′)	Stem (bp)	Loop (nt)	Duplex (bp)
IL-8 HPS1a,b,c	GAG GGT TGT GAT GAA TTC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	10	31	41
IL-8 HPS2a,b,c	GAG GGT TGC TCA GCC CTC TTC AAA AAC TTC TCC ACA ACC CTC	8	26	34
IL-8 HPS3a,b,c	GAG GGT CTC TTC AAA AAC TTC TCC ACA ACC CTC	6	21	27

^aAll the probes were double labeled with 5′-biotin and 3′-fluorescein.

^bHairpin probe design was calculated by MFold free web server (27,28).

^cThe target recognition sequences are listed in italic font. The stem sections of the hairpins are underlined.