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. 2008 May 20;36(11):3802–3818. doi: 10.1093/nar/gkn271

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — STAT5 mRNA levels in Ba/F3-β cells upon STAT5A and STAT5B double (A) and single (B) knockdown. Ba/F3-β cells were transfected either with a non-specific siRNA (ScI), or with siRNAs specific for STAT5A (5A), STAT5B (5B) or both (5AB). Twenty-six hours later, cells were starved for 5 h and stimulated with IL-3. Cells were harvested at the indicated times, and mRNAs levels were monitored by RT-qPCR. A 60% reduction in STAT5 mRNA levels was detected in both the single and double knockdown, with the expected specificity. Reduction in STAT5 protein levels was verified by western blot and has been already published (46). STAT3 mRNA levels (this figure), as well as protein levels (46), remained unaffected, confirming the specificity of STAT5 targeting.