Figure 5.

C3ar1, a novel STAT5 target gene, is overexpressed in (A, C) renal cell carcinoma and (B) melanoma. Expression of C3ar1 and c-Myc was evaluated by RT-qPCR on a panel of cDNAs samples from various human tumors and the corresponding NAT, as well as from normal tissues, as described in Materials and Methods section. mRNA levels were normalized to ubiquitin mRNA levels. C3ar1 is overexpressed in renal cell carcinomas (n = 35) (A) and melanoma tumors (n = 94) (B) compared to normal tissues [renal (n = 12) and skin (n = 15), respectively] and NAT [NAT renal (n = 33) and NAT skin (n = 91), respectively]. P-values for the difference in medians between tumors, normal tissues and NAT are <0.0001 by a two-tailed non-parametric Mann-Whitney test (Graph Pad Prism). The box represents 25–75th percentile of the data, the line represents the median and whiskers are the minimum and maximum values. (C) Expression of C3ar1 and c-Myc is upregulated in renal cell carcinoma (black dots) compared to NAT (gray dots) and normal tissues (white dots). Additionally, expression of C3ar1 and c-Myc is correlated in renal tumor and normal tissue. The squared correlation value, R² is 55%.