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Knockdown of RPC6 results in a post-transcriptional decrease in RPC3 and RPC7 protein levels. (A) Cell lysates were prepared from cells treated with GFP siRNA, RPC6 si1 siRNA, RPC6 si2 siRNA or mock-transfected with the siRNA dilutant (mock). These extracts were resolved on SDS-polyacrylamide gels and analysed by western immunoblotting with antibodies specific to the indicated proteins. (B) RT-PCR analysis of the indicated transcripts from cells treated as above.
