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RNAi of RPC6 specifically compromises pol III transcription in vitro and in vivo. (A) Whole-cell extracts (20 μg) from cells transfected with GFP siRNA, RPC6 si1 siRNA or RPC6 si2 siRNA were used to transcribe tRNALeu, and pre-rRNA templates (250 ng). NS = template-independent, non-specific band. (B) RT-PCR analysis of the indicated transcripts from cells treated as in Figure 1.
