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. 2008 May 17;36(11):3757–3764. doi: 10.1093/nar/gkn272

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — RNAi of RPC6 specifically compromises association of Brf1 with polIII. (A) Cell lysates were prepared from cells treated with GFP siRNA, RPC6 si1 siRNA, RPC6 si2 siRNA or mock-transfected with the siRNA dilutant (mock). These extracts were resolved on SDS-polyacrylamide gels and analysed by western immunoblotting with antibodies specific to the indicated proteins. (B) Extracts (500 μg) prepared from cells treated with GFP siRNA or RPC6 si2 siRNA were immunoprecipitated with normal mouse IgG (lanes 1 and 2) or anti-RPB8 (lanes 3 and 4). Precipitates were resolved by SDS-PAGE and then analysed by western blotting with antibodies to Brf1, RPC1 and RPB8 antibodies.