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. 2008 May 17;36(11):3757–3764. doi: 10.1093/nar/gkn272

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© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — RNAi of RPC6 compromises crosslinking of pol III but not pol II to target genes in vivo. (A) ChIP assay showing crosslinking of cyclin D1, RPC6, and RPB8 to tRNA^Leu, 5S rRNA and 7SK genes and the cyclin D2 promoter in cells transfected with GFP siRNA (G), RPC6 si1 siRNA (1) or RPC6 si2 siRNA (2). (B) PCR products from (A) and an additional independent experiment were quantified by densitometry and normalized to the appropriate input. The average decrease in polymerase crosslinking relative to GFP siRNA is shown for 5S rRNA, tRNA^Leu and 7SK genes and the cyclin D2 gene promoter after treatment with siRNAs against RPC6 (results for si1 and si2 are combined). Error bars indicate the standard deviation from the mean.