Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 May 3;36(11):3579–3589. doi: 10.1093/nar/gkn236

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2008 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 2. — Nitrocellulose filter-binding assays between truncated Tudor-SN mutants and RNA. (A) The binding assays show that TSN, TSN-90, TSN-70 and TSN64 all bind the 20-bp IIUI-dsRNA with comparable affinity, whereas TSN-50 and TSN-25 cannot bind RNA. (B) The filter-binding assays between Tudor-SN proteins and the 20-bp AAUA-dsRNA show that the truncated proteins containing more tandem repeats of SN domains bind AAUA-dsRNA better. TSN-50 and TSN-25 did not bind AAUA-dsRNA. (C) SDS–PAGE analysis of purified TSN-SN34. (D) The filter binding assays between TSN-SN34 and RNA. (E) The summary of the apparent dissociation constants (Kd_app) between Tudor-SN proteins and 20-bp IIUI- and AAUA-dsRNAs.