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RT–PCR analysis of transcription in H4 R45 mutants. RT–PCR analysis was performed on total RNA from wt, H4 R45C and H4 R45H cells using primers specific for the RPB2 and HML genes, as described in Materials and methods section. The RT–PCR product of ACTIN was used as a loading control to normalize the transcript levels of RPB2 and HMLα1 from each strain. The normalized value for the wt cells was set as 1.0.
