Fig. 4. Activity of the principle hits in a cell culture model of Cryptosporidium infection.
(A) Representative cytoxocity assay showing the cytolytic effects of compounds G and H monitored by the release of lactate dehydrogenase (LDH). (B) Parasite proliferation as measured by RT-PCR over 48 hours, the period over which the anti-parasitic effects of compounds A-K were determined. (C) VVL-ELISA C. parvum growth assay; (D) RT-PCR C. parvum growth assay. Assays as described in Materials and Methods.