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. 2008 Jul 1;118(8):2711–2721. doi: 10.1172/JCI34780

Figure 5. Changes in KLF6-SV1 metastatic tumor behavior correlates with markers of cellular proliferation, angiogenesis, and apoptosis.

Figure 5

Metastatic tumors were analyzed for their expression of PCNA, CD31, and TUNEL. (A) Immunohistochemistry for PCNA in pBABE vector– and KLF6-SV1–derived tumors. KLF6-SV1 caused increased PCNA staining in vivo. Eight independent high-power fields for each pBABE (n = 4) and KLF6-SV1 (n = 6) tumor were counted, assessing both the total number of cells and PCNA-positive cells. The graph represents the average percentage of PCNA-positive cells for each group. (B) CD31 staining of pBABE and KLF6-SV1 cell line–derived tumors. Overexpression of KLF6-SV1 protein caused a 4-fold increase in microvessel density (MVD), as measured by the number of CD31-positive endothelial cells per ×400 high-power field (HPF). (C) TUNEL staining of pBABE and KLF6-SV1 tumors. Representative images are shown. Overexpression of KLF6-SV1 decreased apoptosis by 70%. For each tumor, 6 high-power fields were counted and the total number of TUNEL-positive cells was determined. *P < 0.01, **P < 0.001 versus control. Original magnification, ×400.