Skip to main content
. Author manuscript; available in PMC: 2009 Jun 27.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Apr 22;371(2):304–308. doi: 10.1016/j.bbrc.2008.04.063

Figure 1. Effect of MAP kinase inhibition on TPA induced Fra-1expression.

Figure 1

(A) Cells were stimulated with TPA for 0–120 min, cell extracts were isolated, and immunoblotted and JNK1/2, ERK1/2 and p38 kinase activation was analyzed by phosphospecific or native antibodies. (B) Cells were incubated with UO126 (UO) or SP600125 (SP6) for 30 min and then treated with DMSO (DM) or TPA for 3 h. Total RNA was isolated and Fra-1 expression was analyzed by Northern blot analysis. A representative blot of two independent experiments is shown. (C) Cells were incubated with UO126 (UO), SB203580 (SB), or SP600125 (SP6) inhibitors as in panel B for 30 min and then treated with TPA for 90 min, and Fra-1 mRNA expression was analyzed by real-time RT-PCR. Bars represent the mean ± SD (n = 3). * = p<0.001 (D) Cells were transfected with 379-Luc along with the pRL-TK. Cells were serum-starved for 24 h and then stimulated without or with TPA for 5 h. The promoter activity of the 379-Luc under basal condition (control) was set to one. Data represent the mean ± SD (n = 3). * = p<0.03.