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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Mech Ageing Dev. 2008 Feb 6;129(5):282–290. doi: 10.1016/j.mad.2008.01.006

Fig. 4.

Fig. 4

Changes in DLDH diaphorase activity and dehydrogenase activity during postnatal development in heart, kidney and liver. DLDH protein content was determined as described for Fig. 1. DLDH diaphorase activity was measured by the activity staining method described for Fig 2. DLDH dehydrogenase activity was measured spectrophotometrically by following the increase of NADH absorbance at 340 nm in the presence of dihydrolipoamide. Both diaphorase activity and dehydrogenase activity were normalized based on the corresponding DLDH expression and are expressed as a percentage of the values for 10-day-old pups. *p < 0.05 (Welch’s t test) for 60 vs. 30 days of age for heart and kidney and for 20 vs. 10 days for liver.