Table 1.
Name | Sequence 5′–3′ | Fa | Rb | Location | Usage |
---|---|---|---|---|---|
5hRDH12-1 | TCTCTGTGACTTATGCCTATATCTGTCC | ✓ | Promoter region | Amplify PCR fragment a (Fig. 1) | |
3hRDH12-2 | CAGCAAATGTGTACTAACTACCGACTCT | ✓ | Intron 3 | Amplify PCR fragment a (Fig. 1) | |
5hRDH12-4 | CCCAAGCTCACTTACTATACCTCCTTTA | ✓ | Intron 2 | Amplify PCR fragment b (Fig. 1) | |
3hRDH12-4 | CTATAGGTTGAGCATCCCTTATCAGAA | ✓ | Intron 6 | Amplify PCR fragment b (Fig. 1) | |
5hRDH12-5 | AATGAGCAACTAGAGTCTGGGAGTAAAG | ✓ | Intron 6 | Amplify PCR fragment c (Fig. 1) | |
3hRDH12-7 | GACACTACAGAGAGGTGAGATGTAGC | ✓ | After exon 7 | Amplify PCR fragment c (Fig. 1) | |
f6-2 | TTCCATCTTTGCAGTAGAGGTGG | ✓ | Promoter region | Sequencing | |
r2 | TCCTGGGTGCTGAGCAGCAGC | ✓ | Exon 1 | Sequencing | |
f11-3 | CTAAAAGGAGATACTAAGTGACAT | ✓ | Intron 2 | Sequencing | |
r1 | CACAGTAGTACTGTTCAGTCTTC | ✓ | Intron 2 | Sequencing | |
f13-2 | CCCAGTGACAATGCTTATTGG | ✓ | Intron 3 | Sequencing | |
f15-2 | GAGGAATCCACAAACTCAGACC | ✓ | Intron 4 | Sequencing | |
f20 | TGAAATAGAAGGACCTCCGAACC | ✓ | Intron 5 | Sequencing | |
f29-2 | TTAGACCTGGTACTAGATCTTG | ✓ | Intron 6 | Sequencing | |
f30-2 | GCCAGCTGGTGCTGCGAATCC | ✓ | Exon 7 | Sequencing |
F, forward.
R, reverse.