. Author manuscript; available in PMC: 2008 Jun 30.

Published in final edited form as: Vision Res. 2007 May 21;47(15):2055–2066. doi: 10.1016/j.visres.2007.04.005

Table 2.

Primers for control population screening

Mutations screened	Primer sequence	Product size (bp)	MgCl₂ (mM)	Tm (°C)	Cycles	Other
Exon 2 (G46G/T49M)	CCGATAAACAGGCGACTACCTTTGGAAACACAAAGCAATATGGACA	400	1.0	66	35	Q solution
Exon 5 C201R (binds to mutant T)	TGTTGCAAAAAGCTACTGTGAAGCAGGGGTTTTGCCGATC	347	1.0	57	39
Exon 5 A177V	TGTTGCAAAAAGCTACTGTGAGTTAATGTGTTCTCGGTAGT	223	1.0	54	51
Exon 5 R160Q	AATTGGTTCACACCCAGAAGATGACTTCCCAAGTTCCTGTG	398	1.0	66	35
Exon 5 for sequencing	TGTTGCAAAAAGCTACTGTGAAATTGGTTCACACCCAGAAGA	594	1.0	57	39
Exon 6 c.806delCCCTG	CTTCTCCCCCTTTGTCAAGATCATCAGGCACAAACTCAGC	199	1.0	56	38