Fig. 2.

Fluid collection system assembly. The collection pipette was tightened in a pipette holder that was held with the syringe clamps on the micropump (UMP2, World Precision Instrument, FL). The plunger button holder of the micropump pulled the plunger and the withdrawal speed was control by a Micro 4™ controller that produced a suction volume of 2 μL/min. The micropump was mounted on a micromanipulator that was 45° toward the chamber plane.