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. Author manuscript; available in PMC: 2008 Nov 15.
Published in final edited form as: J Immunol. 2007 Nov 15;179(10):6403–6409. doi: 10.4049/jimmunol.179.10.6403

Figure 1.

Figure 1

Distribution of proteins between the Brij-58 resistant (cortical actin) and Brij-58 soluble fractions in CD4 T cells from young and old mice. Purified CD4 T cells from young and old CB6F1 donors were stimulated (+) or not stimulated (-) with anti-CD3 plus anti-CD4 and anti-CD28 crosslinking for 5 min. The cells were lysed with 1% Brij-58 detergent and centrifuged. Samples from the pellet (Brij-Resistant) or soluble (Brij-Soluble) fractions were analyzed by western blots using specific antibodies. In the ERM and p-ERM doublets, moesin is the lower band and ezrin the upper band in each case. The figure is representative of 5 experiments.