3.

Global mapping of the ligand-induced alterations in deuteration onto α/β-tubulin oriented as in a microtubule, with polarity indicated. (a) Epothilone A induced changes modeled on PDB 1TVK, (b) docetaxel induced changes modeled on PDB 1JFF, and (c) peloruside A induced changes modeled on PDB 1JFF. Red indicates statistically significant reductions in labeling upon binding, blue indicates statistically significant increases in labeling upon binding (see methods). Tubulin monomers are labeled in pale cyan (α) and pale green (β). The exchangeable (β-tubulin) and non-exchangeable nucleotides (α-tubulin) are labelled in yellow, and ligands in yellow spheres. The rectangle highlights the proposed peloruside A binding site, which can be found at higher magnification in Fig. 8.