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. 2008 May 20;98(12):1985–1992. doi: 10.1038/sj.bjc.6604398

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Copyright © 2008 Cancer Research UK

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Confirmation of expression in transfected SEG1 cells. SEG1 cells were transfected with pcDNA3.1/Zeo-MYCER, pcDNA3-MAD1 or the corresponding empty vector (VO); in the case of MYCER the chimeric product was then activated by the addition of 4-OHT. (A) qRT–PCR was utilised to assess MYC () or MXD1 () mRNA expression. (B) Western blotting demonstrated the expression the chimeric protein in SEG1-MYCER or MAD1 in SEG1-MAD1. Densitometric scanning approximated the fold increase in expression; a representative blot is also shown. Values represent the mean of two experiments each performed in triplicate ±1 s.e.m. ^* denotes statistical significance (P<0.05).

Inline graphic — Confirmation of expression in transfected SEG1 cells. SEG1 cells were transfected with pcDNA3.1/Zeo-MYCER, pcDNA3-MAD1 or the corresponding empty vector (VO); in the case of MYCER the chimeric product was then activated by the addition of 4-OHT. (A) qRT–PCR was utilised to assess MYC () or MXD1 () mRNA expression. (B) Western blotting demonstrated the expression the chimeric protein in SEG1-MYCER or MAD1 in SEG1-MAD1. Densitometric scanning approximated the fold increase in expression; a representative blot is also shown. Values represent the mean of two experiments each performed in triplicate ±1 s.e.m. ^* denotes statistical significance (P<0.05).