FIGURE 6.

LARP-1 protein accumulates in cytoplasmic granules but does not colocalize with the PGL-1 marker of P granules. (A) Dissected wild-type germ line costained with αLARP-1 (red) and DAPI (blue). Oocytes are indicated by asterisks. LARP-1 is found throughout the germline cytoplasm of the distal arm but tapers off in maturing oocytes (arrowheads). (B–D) Wild-type germ line costained with αLARP-1 (red) (B, D), αPGL-1 (green) (C, D), and DAPI (blue) (D). (D) The merged image. Confocal images were taken of the mid-pachytene region. Arrows point to LARP-1 perinuclear foci; arrowheads point to PGL-1 perinuclear foci. Virtually no yellow is observed in the merged image. (E–G) larp-1(0) mutant pachytene region treated identically as in B–D and confocal images taken with the same settings at the same magnification.