FIGURE 7.

LARP-1 colocalizes with P body proteins in the distal germ line. Wild-type (A–C, E–G) and larp-1(0) (D, H) germ lines costained with αLARP-1 (red) and αDCAP-1 (green). Confocal images were taken of the late mitotic region/early transition zone (A–D) and mid-pachytene region (E–H). Arrows point to foci that stain with both αLARP-1 and αDCAP-1, which are yellow in the merged images. Arrowheads point to foci specific for αDCAP-1 staining; double arrows point to foci specific for αLARP-1 staining. Wild-type (I–K) and larp-1(0) (L) germ lines costained with αLARP-1 (red) and αCAR-1 (green). Arrows point to foci that stain with both αLARP-1 and αCAR-1, which are yellow in the merged image. Arrowheads point to foci specific for αCAR-1 staining; double arrows point to foci specific for αLARP-1 staining. In all cases, wild-type and larp-1(0) germ lines were treated identically and confocal images taken with the same settings at the same magnification.