Figure 5.

WT human ASL binding studies with adenosine phosphonobutyric acid, 2′(3′), 5′-diphosphate (APBADP). The results are replotted as a Scatchard plot, as described in Materials and Methods. Enzyme at 20 μM subunit concentration was incubated with 3–50 μM total APBADP, and the free APBADP concentration was determined as described in Materials and Methods.