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SBP length effect on signal enhancement expressed in terms of SBP injection time for a sample containing 50 nM FITC and 100 nM FL, detected at 4.0 cm. Conditions 1, 2, 3 and 4 were with 10-, 15-, 20- and 25-s SBP injection time, respectively; the FASI duration was 2 s less than the corresponding SBP injection time. Peak a is FITC and peak b is FL.
