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. 2008 May 22;7:16. doi: 10.1186/1475-2859-7-16

Figure 4.

Figure 4

Examples of PolyAcrylamide Gel Electrophoresis and activity on 1-naphthyl acetate. Lysis supernatants of E. coli C41 (DE3) pRos, either expressing the recombinant proteins PF#667, PF#962, and PF#169 fused to the thioredoxin (14.6 kDa), or without induction of PF#962 expression (C-NI), were loaded onto polyacrylamide gels. (A) SDS-PAGE 12% stained with Coomassie Blue. LMW: low molecular weight marker (GE Healthcare Life Sciences). (B) Native-PAGE 10% stained with Coomassie Blue. (C) Activity on 1-naphthyl acetate revealed on native-PAGE 10% using Fast Red TR-salt. The arrows point the recombinant proteins of interest.