Table 3.
Part of the data relating to the 23 putative esterases. [see Additional file 1] for complete data.
| P. freudenreichii proteins predicted by AGMIAL | Method 1: Automatic annotation coming from the most homologous protein | Method 2: Exact nomenclature of InterProScan motifs containing the search terms | Predicted molecular weight (kDa) | EMBL accession number of CDS |
| PF#61 | Proline iminopeptidase (PAP) (PIP) (Prolyl aminopeptidase) | esterase, est-lip-thio, a/b-hydrolase | 39.0 | [EMBL: AM944369] |
| PF#169 | Acetyl esterase family enzyme | a/b-hydrolase | 24.9 | [EMBL: AM944370] |
| PF#279 | no | esterase, est-lip-thio, a/b-hydrolase | 46.4 | [EMBL: AM944371] |
| PF#379 | Putative phospholipase | patatin like, FabD/lysophospholipase | 33.7 | [EMBL: AM944372] |
| PF#435 | Peptidase, S9C (Acylaminoacyl-peptidase) family (EC 3.4.-.-) | esterase, est-lip-thio, a/b-hydrolase | 70.3 | [EMBL: AM944373] |
| PF#456 | Putative magnesium or manganese-dependent protein phosphatase | FabD/lysophospholipase-like | 53.0 | [EMBL: AM944374] |
| PF#667 | Putative lysophospholipase (EC 3.1.1.5). 312 bp | esterase, est-lip-thio, a/b-hydrolase | 34.2 | [EMBL: AM944375] |
| PF#774 | Hypothetical protein | no | 42.9 | [EMBL: AM944376] |
| PF#962 | putative lysophospholipase | esterase, est-lip-thio, a/b-hydrolase | 39.9 | [EMBL: AM944377] |
| PF#1420 | Patatin-like phospholipase | FabD/lysophospholipase | 27.9 | [EMBL: AM944378] |
| PF#1509 | Alpha/beta hydrolase fold | esterase, est-lip-thio, a/b-hydrolase | 29.6 | [EMBL: AM944379] |
| PF#1637 | Acyl-CoA thioesterase II (EC 3.1.2.-). | Acyl-coA-thioesterase | 32.2 | [EMBL: AM944380] |
| PF#1655 | Putative esterase | esterase, est-lip-thio, a/b-hydrolase | 29.5 | [EMBL: AM944381] |
| PF#1758* | Putative esterase precursor | esterase, est-lip-thio, a/b-hydrolase | 36.2 | [EMBL: AM944382] |
| PF#1861 | Helicase, C-terminal:Type III restriction enzyme | Phospholipase | 116.7 | [EMBL: AM944384] |
| PF#1882 | Proline iminopeptidase | esterase, est-lip-thio, a/b-hydrolase | 45.6 | [EMBL: AM944385] |
| PF#2042 | Acetyl esterase family enzyme | esterase, est-lip-thio, a/b-hydrolase | 35.2 | [EMBL: AM944386] |
| PF#2416 | Patatin-like phospholipase | Patatin-like phospholipase | 28.5 | [EMBL: AM944387] |
| PF#2462 | Polyphosphate kinase (EC 2.7.4.1) | Phospholipase | 82.4 | [EMBL: AM944388] |
| PF#2652 | Thioesterase family protein | acyl-ACP-thioesterase/thioesterase | 32.5 | [EMBL: AM944389] |
| PF#2887* | Putative esterase precursor | a/b-hydrolase | 14.4 | [EMBL: AM944383] |
| PF#3004 | (Acyl-carrier protein) S-malonyltransferase (EC 2.3.1.39) | FabD/lysophospholipase-like | 33.0 | [EMBL: AM944390] |
| PF#3022 | Homoserine O-acetyltransferase (EC 2.3.1.31) | alpha/beta-Hydrolases | 44.3 | [EMBL: AM944391] |
Twenty-three putative esterases were identified using four methods (see the legend of Figure 2). The results from method 1 and method 2, the predicted molecular weight, and the accession numbers of the CDS in EMBL database are specified for each of the 23 putative esterases identified. Twelve proteins (in bold) contain the GXSXG motif and were selected for the cloning of their CDS. Eleven proteins were not selected for the cloning of their CDS among which the four underlined proteins containing the GXSXG motif but corresponded to other α/β hydrolases and the two proteins indicated by an asterisk seeming to be predicted from pseudogenes belonging to a unique CDS truncated by a mutation (see text for details).