Figure 7.

N-terminal truncation is required for functional expression in yeast of specific Lotus japonicus aquaporin homologs. Δfps1 Δacr3 Δycf1 transformed with the empty vector pYES2.1 (control) or rAQP9, LjNIP5;1, Ljnip5;1Δ_2–59, LjNIP6;1 or Ljnip6;1Δ_2–72 were spotted at an A₆₀₀ of 0.01 on medium containing various concentrations of (A) As(III) or (B) As(V) as indicated. Growth was recorded after 4 days at 30°C.