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. 2008 Jun 24;8:84. doi: 10.1186/1471-2334-8-84

Table 1.

Primers of biomarkers, and their source, PCR conditions, and expected size.

Targets Primer sequence 5'-3' Annealing temperature Cycle number Expected size (bp) Reference
TGF-β1 sens-AGACGGAATACAGGGCTT TCGATT CA 55°C 35 492 [73]
antisens-CTTGGGCTTGCGACCCAGTAGTA
GFAP sens-GAATGGCCACTAAGGCAGTC 58°C 35 400 [74]
antisens-TGCACTCCCTCTCTCCTGTT
S100B sens-GACTCCAGCAGCAAAGGTGAC 58°C 35 211 [75]
antisens-CATCTTCGTCCAGCGTCTCCA
tTG sens-AGGCCAACCACCTGAACAAA 60°C 35 475 [76]
antisens-CATACAGGGGATCGGAAAGT
NF-L sens-AGCAGAATGCAGACATTAGCG CC 57°C 38 200 [77]
antisens-TGGTCTCTTCGCCTTCC AAGA GT
AβPP69 sens-GCACTAACTTGCACGACTAT GGCATGCTGCTGCCCTG 70°C 35 401
antisens-GCTGGCTGCCGTCGTGG GAACTCGGACTACCTCCTCCAC A
AβPP75 1 sens-CTACCACTGAGTCTGTGGAG 64°C 45 222 [50]
antisens-GCTGGCTGCCGTCGTGG GAAACACGCTGCCACACACCGC C
AβPP77 0 sens-CTACCACTGAGTCTGTGGAG 64°C 45 242
antisens-CTTGAGTAAACTTTGGGA TGACACGCTGCCACACACCGCC
GAPDH sens-ACCACAGTCCATGCCATCAC 60°C 27 452 [75]
antisens-TCCACCACCCTGTTGCTG TA