. 2008 Jun 11;8:92. doi: 10.1186/1471-2180-8-92

Table 1.

Bacterial strains and plasmids used in this study.

Strains and Plasmids	Relevant characteristics and plasmids contruction or source	Reference
Strains
P. fluorescens ST	Sty⁺	[39]
E. coli DH5α	endA1 hsdR17 supE44 thi-1 recA1 gyrA96 (Nal^R) relA1Δ (lacIZYA-argF)U169 deoR(Φ80dlacZΔM15)	Bethesda Res. Lab.
Plasmids
pBluescript II KS+	Cloning vector; Ap^R; 2.9 Kb.	Stratagene
pPR9TT	lacZ promoter probe vector; Ap^R; Cm^R.	[40]
pTE50	pTZ19R derivative containing a P. fluorescens ST chromosomal fragment carrying the genes coding for styR, styA and styB; Ap^R.	[7]
pRK2013	Helper plasmid; ColE1 replicon; Mob+; Tra+; Km^R.	[37]
pSTY1/2	155 bp fragment, encompassing nucleotides -145 to -8, with respect to the styA transcription start point ligated to the EcoRI-PstI sites of pBluescript II KS+; Ap^R.	[16]
pPR9Pa1	355 bp fragment, encompassing nucleotides -115 to +240, with respect to the styA transcription start point, ligated to the XhoI-BamHI sites of pPR9TT; Ap^R; Cm^R.	[20]
pPR9STY1mut	Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -97 and -96 from the styA trascription start point; Ap^R; Cm^R.	This study
pPR9IHFmut	Same construct as pPR9Pa1, but the cloned sequence carries a double mutation in positions -101 and -91 from the styA trascription start point; Ap^R; Cm^R.	This study