Table 1.
Peak INa density, the percentage of late normalized to peak, and sodium channel kinetics from n experiments averaged and reported as mean ± SEM
Samples | Peak INa |
Activation |
Inactivation |
INaL |
||||||
---|---|---|---|---|---|---|---|---|---|---|
pA/pF | n | V1/2, mV | K | n | V1/2, mV | K | n | % | n | |
SCN5A | −233 ± 38 | 9 | −40 ± 2 | 4 | 9 | −77 ± 2 | 5 | 9 | 0.34 ± 0.09 | 5 |
SNTA-WT | −238 ± 33 | 10 | −44 ± 2 | 4 | 7 | −76 ± 2 | 5 | 9 | 0.16 ± 0.04 | 9 |
SNTA-A390V | −393 ± 39* | 16 | −41 ± 2 | 4 | 9 | −69 ± 2* | 6 | 15 | 0.62 ± 0.29 | 18 |
SNTA-A390V + l-NMMA | −336 ± 37* | 6 | −44 ± 2 | 4 | 6 | −75 ± 2 | 5 | 6 | 0.15 ± 0.04 | 4 |
SNTA-WT + NOR3 | −400 ± 67* | 12 | −47 ± 1 | 4 | 11 | −78 ± 1 | 5 | 12 | 0.31 ± 0.07 | 12 |
SNTA-WT + GSNO | −427 ± 151* | 5 | −39 ± 2 | 4 | 3 | −77 ± 3 | 5 | 5 | 0.54 ± 0.22* | 5 |
The parameter fits were obtained from individual experiments with Boltzmann or exponential fits (see Materials and Methods).
*P < 0.05 versus SNTA-WT.