Table 1.
Effect of Dietary Treatment on Polyunsaturated Fatty Acid Brain Content
|
Diet content |
Frontal cortex content |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse Diet Groups | n | DHA %w/w | n-6/n-3 ratio | Chol ppm | Total fatty acid %w/w | Tg(−) DHA(1) | Tg(−) DPA(1) | Tg(−) ARA(1) | Tg(+) DHA(1) | Tg(+) DPA(1) | Tg(+) ARA(1) |
| A) Control | 8 | 0.09 | 7:1 | 290 | 11 | 19.2±0.3 | 0.52±0.02 | 9.6±0.1 | 19.5±0.5 | 0.59±0.03 | 9.5±0.1 |
| B) Low n-3 PFA (low DHA) | 6 | <0.01 | 85:1 | 750 | 6.1 | 18.0±0.5 | 1.43±0.12†† | 9.8±0.1 | 16.4±0.8††¶ | 1.81±0.20†† | 9.9±0.2 |
| C) Low n-3 + DHA (High DHA) | 6 | 0.60 | 5:1 | 750 | 6.1 | 20.5±0.5○○ | 0.29±0.05○○ | 8.4±0.2○○ | 21.3±0.6○○ | 0.22±0.02○○ | 7.9±0.2○○ |
Control diet contained 2.45% and 0.18% of 18:2n-6 (linoleic acid) and 18:3n-3 (linolenic acid), respectively. Low n-3 PFA diet contained 4.86% and 0.06% of 18:2n-6 and 18:3n-3, respectively. Diet C is identical to diet B except for the added DHA. See Experimental Procedures for more details on the diet. ARA, arachidonic acid [20:4(n-6)]; Chol, cholesterol; DHA, docosahexaenoic acid [22:6(n-3)]; DPA, docosapentaenoic acid [22:5(n-6)]; PFA, polyunsaturated fatty acids.
Expressed as mean percentage of total fatty acid ± SEM. Total fatty acids were similar between groups, and values ± SEM were 31.7 ± 0.7, 32.6 ± 0.8, 32.8 ± 1.0, 32.0 ± 0.8, 34.0 ± 0.6, and 32.8 ± 0.7 μg/mg of tissue for Tg(−) control, Tg(−) Low n-3 PFA, Tg(−) Low n-3 PFA + DHA, Tg(+) control, Tg(+) Low n-3 PFA, and Tg(+) Low n-3 PFA + DHA, respectively.
p < 0.01 versus control diet (within transgene).
p < 0.01 versus Low n-3 PFA (within transgene).
p < 0.05 versus Tg(−) (within diet, Low n-3 PFA).