TABLE I.
Effects of Various Reagents on Non-SG Fusion in Excised Patches
| Paired control/testing condition | Amplitude (fF) mean ± SEM (n) |
Ratio of active patchesa mean ± SEM (n) |
Rate constant(1/s)ab mean±SEM (n) |
|---|---|---|---|
| TeTx E234Q | 209.2 ± 60 (9) | 0.89 ± 0.11 (9) | 0.3 ± 0.04 (7) |
| TeTx WT | 10.9 ± 8.4 (10)d | 0.1 ± 0.1 (10)e | 0.36 (1) |
| ATP/GTP | 160.8 ± 42.2 (18) | 0.67 ± 0.11 (18) | 1.52 ± 0.27 (12) |
| NEM/ATP/GTP | 138.5 ± 43.7 (18) | 0.5 ± 0.12 (18) | 1.01 ± 0.21 (9) |
| ATP | 119.8 ± 31.8 (12) | 0.58 ± 0.15 (12) | 0.8 ± 0.11 (7) |
| AMP-PNP | 35.3 ± 9.4 (13)c | 0.15 ± 0.1 (13)c | 1.04 ± 0.27 (3) |
| Mg2+ buffer | 53.3 ± 19.9 (10) | 0.3 ± 0.15 (10) | 1.12 ± 2e-3 (2) |
| EDTA | 168.6 ± 70.7 (10) | 0.8 ± 0.13 (10)c | 1.5 ± 0.34 (8) |
| EDTA 1min | 221.3 ± 106.2 (5) | 0.8 ± 0.2 (5) | 0.72 ± 0.11 (4) |
| neomycin/EDTA | 102.4 ± 38.5 (5) | 0.8 ± 0.2 (5) | 0.47 ± 0.1 (3) |
| FVPP 1min | 267.3 ± 61 (8) | 1 (8) | 0.75 ± 0.29 (8) |
| PIP2Ab/FVPP | 118.3 ± 25.6 (7) | 0.86 ± 0.14 (7) | 1.12 ± 0.3 (6) |
| FVPP 2min | 78.2 ± 39 (6) | 0.5 ± 0.22 (6) | 0.32 ± 0.09 (2) |
| PIP2Ab/FVPP | 128.9 ± 40.4 (6) | 0.67 ± 0.21 (6) | 0.68 ± 0.17 (4) |
| Mg2+ buffer | 93.1 ± 44.2 (13) | 0.38 ± 0.14 (13) | 1.08 ± 0.35 (5) |
| neomycin | 71 ± 22.9 (14) | 0.29 ± 0.13 (14) | 0.62 ± 0.21 (5) |
| Mg2+ buffer | 103.1 ± 47.3 (14) | 0.43 ± 0.14 (14) | 0.92 ± 0.19 (6) |
| PIP2Ab | 92.6 ± 46.8 (14) | 0.43 ± 0.14 (14) | 1.17 ± 0.13 (5) |
| ATP | 76.6 ± 16.9 (14) | 0.64 ± 0.13 (14) | 1.06 ± 0.23 (9) |
| staurosporine/ATP | 50.3 ± 13.8 (15) | 0.47 ± 0.13 (15) | 1.25 ± 0.34 (7) |
| ATP | 62.2 ± 13.5 (20) | 0.6 ± 0.11 (20) | 0.83 ± 0.17 (11) |
| wort/adeno/ATP | 6.9 ± 3.1 (21)e | 0.05 ± 0.05 (21)e | 0.88 (1) |
| ATP | 47.3 ± 26.4 (8) | 0.38 ± 0.18 (8) | 1.04 ± 0.32 (3) |
| LY294002/ATP | 159.1 ± 70.9 (7) | 0.57 ± 0.2 (7) | 1.49 ± 0.19 (4) |
| ATP | 104.9 ± 75.1 (4) | 0.5 ± 0.29 (4) | 1.87 ± 0.51 (2) |
| PI-TP/ATP | 202 ± 50.4 (4) | 1 (4) | 1.28 ± 0.46 (4) |
TeTx, tetanus toxin light chain, 200 nM; NEM, N-ethylmaleimide, 1 mM; AMP-PNP, 2 mM; neomycin, 500 μM; PIP2Ab, 1:50; staurosporine, 200 nM; wort, wortmannin, 4 μM; adeno, adenosine, 0.5 mM; LY294002, 100 μM; PI-TP, PI-transfer protein, 140 μg/ml.
a
Counts patches with amplitude ≥50 fF.
b
Outliers are removed using Grubbs' test.
c
P<0.05.
d
P<0.01.
e
P<0.001.