Table II.
Primers Used for rtPCR
| Transcript | GenBank/EMBL/DDBJ accession number |
Expected product (bp) |
Primer set (5′ - 3′) | 5′- binding site |
|
|---|---|---|---|---|---|
| NHE1 | NM_016981 | 315 | for | TGTAGAGTAGAAAGTACTG | 4262 |
| rev | TGAAAAAGGTTCAGTTTCC | 4558 | |||
| NHE2 | NM_001033289 | 127 | for | CCATTTCTGTGTTTTTCCTG | 3247 |
| rev | TCTTTAAACACTATGGGCTG | 3354 | |||
| NHE3 | NM_001081060 | 207 | for | AACTATGAAGAGATCAGTGG | 2161 |
| rev | AGAGTTTGGAATCTGAACAC | 2348 | |||
| NHE4 | NM_177084 | 223 | for | TTGCTCTGGAGTTTAGTAAC | 3566 |
| rev | TATTGATCTGGTTGCCATAG | 3769 | |||
| NHE5 | NM_001081332 | 356 | for | TCAGGAGTAGAGAATAGTAG | 4763 |
| rev | CCTTTGTCTAGTAAATGCTC | 5099 | |||
| NHE6 | NM_172780 | 385 | for | GTGAAGAAATGTGTACTACC | 2163 |
| rev | GTTTTTCCAACAAAGGAACC | 2528 | |||
| NHE7 | NM_177353 | 403 | for | GTGTATGATAATCAAGAGCC | 2010 |
| rev | GAGTGGTAAGATAGTTTCAG | 2384 | |||
| NHE8 | NM_148929 | 249 | for | TTCTTTCTTCTCTGACATGG | 4237 |
| NM_178371 | rev | TAACTATCCAGTCATGTGAC | 4466 | ||
| NHE9 | NM_177909 | 226 | for | ACCTTATCACATGTATCCTC | 2212 |
| rev | ATCCAAAATCCACATGAAGG | 2418 |