Figure 2.

The KCNMB3 1a exon is highly conserved in mammals. Segments of nucleotides spanning the putative 1a exon of various placental mammals were aligned using the UCSC genome browser. Displayed nucleotides are complementary to those for mRNA and are displayed in reverse order to that typically used for amino acid alignments. The top two tracks show, respectively, the nucleotide positions on human chr. 3 and the presumptive open reading frame for the identified human 1a exon. The PhastCons plot shows scores for 17 placental mammals plus human over this genome segment. For the alignments, the segment boxed in red indicates the highly conserved consensus GT intron–exon splice site preceding the ORF (reverse complement: GTAAGT). Numbers on the Gaps track indicate number of inserted residues for particular species indicated by vertical orange lines. Double lines indicate segments with one or more unalignable bases. Single lines indicate positions with no alignable bases.