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. 2008 Jul;132(1):29–50. doi: 10.1085/jgp.200709865

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© 2008 Yaradanakul et al.

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Figure 9. — Phosphoinositide, phosphatidate, and total mono/diacylglycerol (M/DAG) levels of permeabilized BHK cells in dependence on ATP, AMP-PNP, and Ca. (A) PIP and PI(4,5)P₂ levels of BHK cells permeabilized with 40 μM β-escin. All results are the average of two to four measurements. Left bars give levels for control cells and permeabilized cells with 2 mM ATP and no Ca (0.5 mM EGTA). The set of three bars in the middle give PIP and PI(4,5)P₂ levels with ATP, without ATP for 5 min, and with 2 mM AMP-PNP for 5 min. The right two bars give phospholipid levels of control cells and cells exposed to 0.4 mM free Ca for 2 min. (B) From left to right, the bar graphs give mono and diacylglycerol (M/DAG) and phosphatidate (PA) levels without and with 0.4 mM free Ca for 1 min (left four bars), without and with 0.3 mM carbachol for 3 min, without and with 0.4 mM free Ca together with 0.3 mM carbachol, and without and with 0.4 mM free Ca for 1 min in the presence of AMP-PNP and no ATP (right four bars).