Table 1. Comparison of allele frequency of three SNPs from a pool of 192 controls using manual and software analysis.
| Gene name: CYP2E1, SNP ID: rs 915906 | ||||||||
| No.of Samples | Individual Samples | Pooled Samples (Software) | Pooled Samples (Manual) | % Error (Software) | % Error (Manual) | |||
| C% | T% | C% | T% | C% | T% | |||
| 1-96 | 18.75 | 81.25 | 18.8 | 81.2 | 18.2 | 81.8 | 0.05(±4.42) | 0.55(±0.063) |
| 1-192 | 16.4 | 83.6 | 14.2 | 85.8 | 15.62 | 84.38 | 2.2(±4.72) | 0.78(±0.042) |
| Gene name: DrD2, SNP ID: rs 6279 | ||||||||
| No.of Samples | Individual Samples | Pooled Samples (Software) | Pooled Samples (Manual) | % of Error (Software) | % of Error (Manual) | |||
| C% | G% | C% | G% | C% | G% | |||
| 1-96 | 28.64 | 71.35 | 30.5 | 69.5 | 29.55 | 70.45 | 1.86(±0.49) | 0.9(±0.065) |
| 1-192 | 31.9 | 68.1 | 36.1 | 63.9 | 33.58 | 66.42 | 4.2(±1.42) | 1.68(±0.034) |
| Gene name: COMT, SNP ID: rs933271 | ||||||||
| No.of Samples | Individual Samples | Pooled Samples (Software) | Pooled Samples (Manual) | % of Error (Software) | % of Error (Manual) | |||
| C% | T% | C% | T% | C% | T% | |||
| 1-96 | 26.04 | 73.96 | 27.98 | 72.02 | 27.59 | 72.41 | 1.94(±6.59) | 1.55(±0.18) |
| 1-192 | 26.04 | 73.96 | 29.25 | 70.75 | 26.59 | 73.4 | 3.21(±4.40) | 0.55(±0.12) |
Manual and software analysis comparison with the reference (true values) for pools of 96 and 192 for three SNP from genes CYP2E1, DrD2 and COMT. The table demonstrates significant lower error rates by manual evaluation.