Table 2. Influenza virus type, results for each qPCR replicate, and exhalation rate.
| qPCR of Filter Extracta | |||||
| Subject ID | Influenza virus type (sub-type) | Replicate 1 | Replicate 2 | Replicate 3 | Influenza virus RNA exhalation rateb |
| A-06 | A (H3) | 47 | 21 | 44 | 20 |
| A-07 | A (H3) | ND | ND | <6 | <3.2 |
| A-08 | B | ND | ND | ND | ND |
| A-11 | B | ND | ND | ND | ND |
| A-21 | A (H3) | ND | ND | ND | ND |
| A-23 | A (H3) | ND | ND | <6 | <3.2 |
| A-24 | B | ND | 7 | ND | <3.2 |
| A-25 | B | ND | ND | ND | ND |
| A-34 | B | ND | ND | ND | ND |
| B-01 | A (H3) | ND | ND | ND | ND |
| B-09 | B | ND | ND | ND | ND |
| B-25 | B | ND | ND | ND | ND |
| A-37 (control) | ND | ND | ND | ND | |
| A-38 (control) | ND | ND | ND | ND | |
a
Number of influenza RNA copies detected per well (5 µl cDNA per well).
b
Influenza virus RNA copies/ minute
ND = not detected by qPCR; limit of quantification was 6 influenza virus RNA copies per qPCR well when all three replicates were detected.