Figure 4.
Nfasc186 rescues the PNS nodal complex in Nfasc−/− mice. (A) FLAG-tagged Nfasc186 can rescue the nodal complex in teased sciatic nerve fibers immunostained for the nodal proteins voltage-gated sodium channels (Nav), βIV-Spectrin, AnkyrinG, and NrCAM. Myelin was visualized using MBP as a marker. (B) NrCAM is present at PNS nodes but absent from CNS nodes. Bars, 5 μm.