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. 2008 Jun 30;181(7):1169–1177. doi: 10.1083/jcb.200712154

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© 2008 Zonta et al.

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Figure 6. — Axoglial adhesion complex promotes oligodendrocyte process migration. (A and B) Axoglial adhesion complexes at the tips of myelinating processes in teased fibers were immunostained for Caspr or Caspr and Nfasc155 (insets) at P4 (A) and P6 (B). Immunostaining for the myelin protein MAG, the axonal marker NF-H, Caspr, and Nfasc155 suggested that oligodendrocyte processes with Neurofascin and Caspr at their tips might converge from P4 to P6 during development. (C–E) Myelinating processes could extend along axons in Nfasc^−/− mice (C) and in Neurofascin-null mice expressing either Nfasc186 (D) or C-terminally truncated Nfasc155 (E). (F) Measurement of the gap between myelinating processes (the interheminodal gap) at P6 showed that the convergence of oligodendrocyte processes in teased fibers from the ventral funiculus of the cervical spinal cord is either retarded or stalled in the mutant, with or without Nfasc186, compared with wild-type nerves at the same age. There was no significant difference in the mean interheminodal gap of mutant fibers at P6 with or without Nfasc186, but each was significantly larger than the nodal width in either wild-type nerves at P6 or when the axoglial adhesion complex was reconstituted using C-terminally truncated Nfasc155. However, there was no significant difference in the width of the gap in wild-type nerves compared with Neurofascin-null nerves rescued with C-terminally truncated Nfasc155, indicating that this protein effectively rescued process migration. (G) At P16, the interheminodal gap between myelinating processes in nerves from cervical spinal cord lacking an intact axoglial junction is not significantly different from wild type, showing that convergence of myelinating processes is retarded rather than arrested at P6. Values are means ± SEM. n = 3 mice for each condition. *, P < 0.01 (one-way analysis of variance test). (H) Electron microscopy of transverse sections from the cervical cord of 16-d old Nfasc^+/+ and Nfasc^−/−/Nfasc186 animals showed extensive myelination in the rescued mutant cord. In A and E, the arrowheads show the position of the adhesion complex. In C and D, the arrows show the tips of oligodendrocyte processes. Bars: (A–E) 5 μm; (H) 2 μm.