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RP-HPLC of pear-secreted AGP. Secreted AGP, which had been partially purified by anion-exchange chromatography, was fractionated by RP-HPLC and 10-ml fractions were collected. (Upper) The protein content of each fraction, determined by absorbance at 215 nm in analytical RP-HPLC, is plotted. (Lower) The AGP content of each fraction, determined by Yariv radial diffusion gel assay, is plotted. The shaded regions indicate the fractions that were pooled and used for further study.
