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ESI-MS of deglycosylated PcAGP1. A portion of the pooled RP-HPLC fractions 2 and 3 of pear-secreted AGP (see Fig. 2) was deglycosylated with anhydrous HF and analyzed by ESI-MS. Numbers above each signal refer to the charge state (z) and the m/z value of the most intense ion. Each signal contains several ions separated by 16/z, because of heterogeneity of proline hydroxylation. The molecular weight can be calculated from each ion as (m/z)z-z.
