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. 2008 Jun 30;181(7):1073–1081. doi: 10.1083/jcb.200704079

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© 2008 Vadaie et al.

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Figure 3. — Cleavage of Msb2p induces MAPK signaling and is regulated at the level of YPS1 expression. (A) FUS1-lacZ expression in strains containing Msb2p deletions. (B) Western blot of HA-Msb2p in WT and yps1Δ strains grown in mid-log phase yeast extract peptone dextrose (YEPD) or YEP-GAL (YEPG) medium for 1 and 3 d. Dpm1p was used as a loading control (Ctl). Molecular mass standards (kD) are indicated on the left of the gel blots. (C) YPS1-lacZ expression in cells incubated in mid-log phase in SCD (Glu) or SC-Gal (Gal) medium for 1and 3 d. (D) FUS1-lacZ expression in indicated strains overexpressing YPS1 and MKC7. Error bars represent the standard deviation of the values derived from at least two separate experiments. (E) Cell morphology in strains containing high copy (2 μm) YPS1. Bar, 5 μm.