Table 1. Genes differentially expressed between polygyne S. invicta workers bearing Gp-9 genotypes Bb and BB that significantly match annotated genes in public databasesa.
Fire ant gene | Putative gene product of best matchb | E-value | Gene category | Expression Ratio (Bb/BB)c | P-valued |
SI.CL.18.cl.1888.Contig1e | odorant binding protein homolog #1 (Q5EP09) | 5.00E-10 | odorant binding | 0.49 | 1.17E-04 |
SI.CL.40.cl.4070.Contig1 | venom allergen homolog (P35778) | 6.00E-72 | allergen | 0.53 | 2.88E-04 |
SI.CL.1.cl.162.Contig1e | antennae-specific chemosensory protein homolog (Q2VW29) | 3.00E-17 | odorant binding | 0.54 | 6.71E-06 |
SiJWG04ABQ.scf | S. invicta venom allergen 3 (P35778) | 1.00E-136 | allergen | 0.55 | 1.21E-05 |
SI.CL.5.cl.547.Contig1 | Step ii splicing factor slu7 (Q16FY9) | 1.00E-85 | RNA processing | 0.63 | 4.95E-06 |
SI.CL.24.cl.2429.Contig1 | Proteasome subunit alpha type 2 (Q8T0Y8) | 1.00E-114 | protein degradation | 0.77 | 2.62E-06 |
SI.CL.0.cl.015.Contig2e | mitochondrial Ribosomal protein L21 (Q29DI1) | 6.00E-36 | mitochondrial | 1.41 | 7.27E-05 |
SiJWH01ABW.scf | low density lipoprotein receptor-related protein associated protein (lrpap1) (A2I465) | 2.00E-34 | receptor modulation | 1.57 | 1.15E-11 |
SI.CL.3.cl.385.Contig1 | odorant binding protein homolog #2 (Q8WRP9) | 2.00E-06 | odorant binding | 1.69 | 4.69E-05 |
SiJWC05ACO.scf | growth-arrest-specific protein 8 (ENSCSAVP00000007111) | 2.00E-51 | cytoskeleton regulation | 2.18 | 4.33E-11 |
SiJWC03CAW.scf | TatD related deoxyribonuclease (ENSCINP00000014669) | 9.00E-09 | nucleic acid metabolism | 2.44 | 4.84E-09 |
SI.CL.26.cl.2690.Contig1 | BEL-PAO transposon polyprotein (Q4JS97) | 4.00E-32 | transposon | 3.61 | 5.41E-15 |
SiJWF04BEA.scf | piggyBac transposon (Q75R41) | 6.00E-15 | transposon | 28.94 | 3.10E-27 |
Threshold, E<1e-5. See Table S3 for all BLASTX matches with E≤1 for all 39 differentially expressed genes.
Excludes Ensembl Apis gene predictions. Accession numbers of best matches (TrEMBL, Swiss-Prot, or Ensembl databases) are shown in parentheses.
Expression ratios are based on averages for all Bb and BB workers in the 20 polygyne study colonies. Elevated expression in Bb workers relative to BB workers is highlighted with bold italics.
P-values from ANOVA calculations are averages for genes represented by more than one significantly differentially expressed clone on the microarray (10% FDR).
Assembled sequence is composite of separate contigs that were merged because they have >95% sequence identity.