FIGURE 5.

Characterization of purified Ict1p. A, purified Ict1p was incubated with 100 μm of various acyl acceptors such as Glc-3-P (G3P), LPA, LPC, LPE, and LPS, and the reaction products were resolved on silica-TLC using chloroform/methanol/acetone/acetic acid/water (50:10:20:15:5, v/v) as the solvent system, and corresponding acylated phospholipid products were measured. Values are the average of two separate experiments, and each was performed in triplicate. B, LPA acyltransferase activity was monitored using 50 μm of [³H]LPA and 50 μm of palmitoyl-CoA or oleoyl-CoA or stearoyl-CoA. C, effect of Triton X-100 and CHAPS on LPA acyltransferase activity. Values are the mean of three separate experiments, and each was performed in triplicate.