FIGURE 3.

ΔΨ establishment in control and subunit h-depleted mitochondria. Rhodamine 123-monitored ΔΨ variations were assayed, as described under “Experimental Procedures,” in isolated mitochondria (0.3 mg·ml^-1) from RFY5-1 grown without (left panels) and with doxycycline (Dox) (right panels) in the YPGal medium. A, ΔΨ variations during oxidative phosphorylation. B, ΔΨ establishment by ATP hydrolysis. Additions were 0.3 mg·ml^-1 mitochondria (m), 170 mm ethanol (EtOH), 50 μm ADP, 600 μm KCN, 400 μm ATP, 10 μg·mg^-1 of proteins DCCD and 3 μm CCCP. a.u., arbitrary units.