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. 2008 Apr 11;283(15):9787–9796. doi: 10.1074/jbc.M708839200

FIGURE 3.

FIGURE 3.

AICAR-stimulated PAS-160 phosphorylation is greatest in TA muscle. The time courses of AICAR-stimulated Akt substrate phosphorylation at a molecular weight of 160 (PAS-160) and AMPK Thr-172 phosphorylation (P-AMPK) in vivo were determined by injecting mice with AICAR subcutaneously for 0 (control), 10, 30, or 60 min. PAS-160 and P-AMPK were measured in soleus (A) and tibialis anterior muscle (B) lysates by immunoblotting. α-Tubulin was utilized as a loading control. Maximal PAS-160 (C) and P-AMPK (D) phosphorylation between soleus, TA, and EDL muscle was compared by immunoblotting lysates from the 30-min time points. A paired immunoblot for AS160 is included in panel C. The data are expressed as the means ± S.E. (n = 3-8). a-c, 1-3, groups within each panel not marked by the same letter or number are statistically different. Groups annotated by letters cannot be compared with groups annotated by numbers. *, AICAR stimulation for a given muscle had a statistically significant effect on phosphorylation compared with basal, p < 0.05.