FIGURE 5.

Physical interaction of annexin A2 and Rab14 by co-immunoprecipitation. A, 293A cells were transfected with pEGFP-Rab14 or pEGFP for 48 h. The cell lysate was immunoprecipitated (IP) with rabbit polyclonal anti-GFP antibodies and detected by Western blot using mouse monoclonal anti-GFP and anti-annexin A2 antibodies. IN, input. B, 293A cells were transfected with pEGFP-Rab14 and/or pCMV-AII-p65 for 48 h. The cell lysate was immunoprecipitated (IP) with anti-p65-agarose and analyzed by Western blot using anti-GFP and anti-annexin A2 antibodies. IN, input.