FIGURE 8.

Effects of HSL and ATGL overexpression on net TG synthesis and FA oxidation in McA-RH7777 cells. Cells were infected with AdHSL, AdATGL, or AdGFP for 24 h followed by 16 h of incubation in a conditioned medium containing 0.4 mm OA, 1.5% BSA, and 1 μCi of [¹⁴C]OA. A–F, cellular TG (A), DG (B), CE (C), and PL (D) were separated by TLC and counted in a scintillation counter. Medium was collected, and oxidation of [¹⁴C]OA to [¹⁴C]CO₂ (E) or [¹⁴C]ASM (F) was measured as described under “Experimental Procedures.” Data are presented as means ± S.D. and expressed in counts/min per μg of cellular protein. G, cellular gene expression was assessed by qPCR and normalized to β-actin expression. Levels of mRNA for each gene are expressed as a percentage of those (±S.D.) in AdGFP-infected cells. The experiment was performed in triplicate. Comparisons were made against AdGFP-infected cells or between AdHSL- and AdATGL-infected cells by Student's t test. p values: ^a, = 0.06; ^*, p < 0.05; ^**, p < 0.01, ^***, p < 0.001.