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RFX is the stimulatory factor. A, SDS-PAGE of purified RFX. B, RFX stimulates EXO1-catalyzed excision. Mismatch-provoked DNA excision assay was performed in a reconstituted system containing the presence and absence of recombinant RFX. Assays contained EXO1 (3 fmol), MutSα (400 fmol), MutLα (400 fmol), RPA (800 fmol), and HMGB1 (1,000 fmol) with or without native (800 fmol) or recombinant (400 and 800 fmol) RFX.
