FIGURE 6.
PP2Acα negatively regulates αIIbβ3 activation in murine megakaryocytes. A, Alexa 488 fibrinogen binding in untransfected megakaryocytes. Large and alive megakaryocytes that expressed αIIb were analyzed for fibrinogen binding in response to 2.5 mm PAR4AP in the presence and absence of 10 mm EDTA by flow cytometry. B, expression of PP2Ac in control and PP2Acα-transfected siRNA. The blot was stripped and analyzed for PP1c and actin. These blots are representative of three experiments. C, increased fibrinogen binding in megakaryocytes transfected with murine PP2Acα siRNA. Fibrinogen binding was studied as described in panel A. Results are mean ± S.E. of six experiments, and the increased fibrinogen binding in megakaryocytes transfected with PP2Acα siRNA over the control siRNA was significant at +, p = 0.02 by analysis of variance.