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. 2008 May 9;283(19):12756–12762. doi: 10.1074/jbc.M705003200

FIGURE 1.

FIGURE 1.

A, DNA substrate used in this study. DNA template pUC15RNTS for T7 and RNAP II transcription consisted of closed circular DNA molecules containing the Ig Sμ repeat cloned downstream of the T7 or adenovirus major late promoter (AdLMP), constructed as described under “Experimental Procedures.” The transcription start sites (+1) are represented by bent arrows. B, T7 RNAP transcription of substrates containing G-rich sequences in the transcribed (TS) or non-transcribed (NTS) strand. DNA templates were transcribed in vitro such that the transcripts were radioactively labeled. Elongation was allowed to proceed for 30 min after addition of NTPs to the reaction. Lanes 1 and 3, templates containing G-rich sequences in the transcribed strand; lanes 2 and 4, templates containing G-rich sequences in the non-transcribed strand. G4, transcripts arrested near G4-forming sequences; RO, full-length runoff transcript; M, 100-bp ladder.